Recombinant Periostin(POSTN)

Organism Species: Mus musculus(Mouse)

Instructionmanual

Art.No: BES2339Mu01 

specifications: 50ug

[ PROPERTIES ] 

Source: Prokaryotic expression. 

Host: E. coli 

Residues: Ser500~Leu630 

Tags: N-terminal His-Tag 

Tissue Specificity: Heart. 

Subcellular Location: Secreted, extracellular space, extracellular matrix 

Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 15.9kDa

Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducingconditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles

Store at 2-8 oC for one month.

Aliquot and store at -80 oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37 oC for 48h, and no obvious degradation and precipitationwereobserved.The loss rate is less than 5% within the expiration dateunderappropriate storage condition.

[ SEQUENCE ]

LHDKLRQDKR FSIFLSLLEA ADLKDLLTQP GDWTLFAPTN DAFKGMTSEERELLIGDKNA LQNIILYHLT PGVYIGKGFE PCVTNILKTT QGSKIYLKGVNETLLVNELK SKESDIMTTN GVIHVVDKLL

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE