Recombinant Collagen Type VI Alpha 3 (COL6a3)

Organism Species: Mus musculus (Mouse)

Instruction manual

Art.No: BES23148Mu01

specifications: 50ug/200ug/1mg

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asp2009~Leu2205

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 350µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 26.2kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]

Storage:  Avoid repeated freeze/thaw cycles.

         Store at 2-8ºC for one month.

         Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

DL AFILDSSEAT TLFQFNEMKK YIGYVIRQLD LSPDPKASQH

FARVAvVQQS TYESVDNASV PPVKVEFSLT DYGAKEKLLD FLSRRMTQLQGTMGLGNAIE YTIENIFESA PNPRDLKIMV LMLTGDMQRQ QLEEAQRAILQAKCKGYFFV VLGIGRKVNI KEVYSFASEP NDVFFKFVDK STELNEEPLMRFGRL

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.