Recombinant Collagen Type VI Alpha 3 (COL6a3)
Organism Species: Mus musculus (Mouse)
Instruction manual
Art.No: BES23148Mu01
specifications: 50ug/200ug/1mg
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Asp2009~Leu2205
Tags: N-terminal His Tag
Subcellular Location: Secreted
Purity: > 97%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 350µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 26.2kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
DL AFILDSSEAT TLFQFNEMKK YIGYVIRQLD LSPDPKASQH
FARVAvVQQS TYESVDNASV PPVKVEFSLT DYGAKEKLLD FLSRRMTQLQGTMGLGNAIE YTIENIFESA PNPRDLKIMV LMLTGDMQRQ QLEEAQRAILQAKCKGYFFV VLGIGRKVNI KEVYSFASEP NDVFFKFVDK STELNEEPLMRFGRL
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.